PIRA-PCR
Source sequence (up to 1,000 bases)
Position of SNP from start of sequence
Allele 1
Allele 2
Optimum primer size
Maximum primer size
Minimum primer size
Optimum product size
Maximum product size
Minimum product size
Optimum primer Tm
Maximum primer Tm
Minimum primer Tm
Maximum primer GC%
Minimum primer GC%
Maximum complementarity
Maximum 3' complementarity
Salt concentration (mM)
Annealing primer concentration (nM)
Number of bases for mismatches on either side of SNP
Number of primer pairs
Help
For any questions or comments, please contact xke@soton.ac.uk
Please cite the following paper in your resulting publications of using this program:
Xiayi Ke, Andrew Collins and Shu Ye
PIRA PCR designer for restriction analysis of single nucleotide polymorphisms.
Bioinformatics, 2001, Vol.17, No.9, Pages 838-839