PRIMER1: primer design for tetra-primer ARMS-PCR
Source sequence (up to 1,000 bases)
Position of SNP from start of sequence
Allele 1
Allele 2
Optimum primer size
Maximum primer size
Minimum primer size
Optimum (inner) product size
Maximum (inner) product size
Minimum (inner) product size
Maximum relative size difference of two inner products
Minimum relative size difference of two inner products
Optimum primer Tm
Maximum primer Tm
Minimum primer Tm
Maximum primer GC%
Minimum primer GC%
Maximum complementarity
Maximum 3' complementarity
Salt concentration (mM)
Annealing primer concentration (nM)
Number of outputs
Help
For any questions or comments, please contact arc@soton.ac.uk
References:
Andrew Collins, Xiayi Ke
Primer1: primer design web service for tetra-primer ARMS-PCR.
(Article submitted for publication)
Shu Ye, Sahar Dhillon, Xiayi Ke, Andrew R.Collins and Ian N.M.Day
An efficient procedure for genotyping single nucleotide polymorphisms.
Nucleic Acid Research, 2001, Vol. 29, No. 17, E88-8