PCR Designer for Restriction Analysis of Sequence Mutations
Source sequence (up to 1,000 bases)
Starting position of mutation
Allele1
Allele2
Optimum primer size
Maximum primer size
Minimum primer size
Optimum product size
Maximum product size
Minimum product size
Optimum primer Tm
Maximum primer Tm
Minimum primer Tm
Maximum primer GC%
Minimum primer GC%
Maximum complementarity
Maximum 3' complementarity
Salt concentration (mM)
Annealing primer concentration (nM)
Number of bases for mismatches on either side of mutation
Number of restriction sites
Number of primer pairs
Help
For computing questions, please contact xiayi@well.ox.ac.uk
For questions related to laboratory applications, please contact Shu.Ye@soton.ac.uk
Please cite the following paper in your resulting publications of using this program:
Xiayi Ke, Andrew R. Collins, Shu Ye (2002) PCR Designer for Restriction Analysis of Various Types of Sequence Mutation. Bioinformatics 18: 1688-1689.